How to Use Cytopathology Services

On-site prepared slides must be identified with the patient's full name and medical record number and/or date of birth written with lead pencil on the frosted glass slide.

Immediate wet fixation is absolutely essential for cytologic samples that are stained by the Papanicolaou method.  Even the slightest delay in fixation will cause air-drying, resulting in poor cellular detail.  Air-dried smears are good only for diff-quick stain.  Spray fixative can be ordered in Peoplesoft #167826.  As soon as the smear is prepared, spray fix the smear within two to three seconds.  Hold the spray bottle (non-aerosol) three to four inches away from the slide.  Pump three to four times or until the slide is flooded.  Once the fixative on the smear is dry, place the slide in a cardboard slide folder (damp/wet slides will adhere to holder and be unsatisfactory for evaluation).

Liquid fixed specimens; e.g., washings, rinses, fine needle aspirates, etc.: Each specimen should be placed in a separate container or tube with Cytolyt solution (available from the Cytopathology Laboratory, Detroit campus, (313) 916-2328 or in Peoplesoft #258791) or Saccomanno fixative if the specimen is a transbronchial fine needle aspiration (available from the Cytopathology Laboratory, Detroit campus, (313)916-2328 or in Peoplesoft).  The container/tube must be labeled with patient's name, medical record number, and specimen description.

Fresh specimens: Prompt delivery of fresh, unfixed material to the Cytopathology Laboratory is critical.  Leaving the specimens at room temperature will result in rapid deterioration of cellular material. Such specimens are unsatisfactory for cytopathologic evaluation. Add ice packs in with these specimens and refrigerate if unable to deliver promptly to minimize degeneration. Off-site locations sending glass containers must pad the containers to avoid breakage.

Complete the cytology order and ensure it has been collected in Epic. Both specimen and laboratory requisition must be delivered in biohazard specimen transport bag with specimen placed in the sealed portion of the bag and the laboratory requisition in the outside pocket.

For any questions regarding specimen collection, specimen preservation, or procurement supply orders, contact Cytopathology Laboratory at 313-916-2328.